TABLE 2.
Percentage of carbon formed from labeled substrate in medium
TG-Palmitate |
TG-Glycerol |
|||
Substrate | 5 mM Glucose | 17.5 mM Glucose | 5 mM Glucose | 17.5 mM Glucose |
% carbon | % carbon | % carbon | % carbon | |
1 mM [1-13C]acetate | 16.6 ± 1.7a | ND | ND | ND |
0.5 mM [U-13C]pyruvate | 12.3 ± 2.3 | 12.3 ± 2.6 | 12.5 ± 1.1 | 13.9 ± 0.6 |
[U-13C]glucose | 42.4 ± 2.0 | 47.0 ± 1.2b | 72.1 ± 4.6 | 72.6 ± 5.1 |
2 mM [U-13C]glutamine | 10.4 ± 0.7 | 9.2 ± 0.6 | 14.4 ± 0.5 | 13.4 ± 0.9 |
ND, not determined.
Because [1-13C]acetate was used, this is equivalent to 33.2% of TG-palmitate arising from medium acetate.
Significantly different from 5 mM [U-13C]glucose (P < 0.05).