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. 2011 Sep;52(9):1683–1692. doi: 10.1194/jlr.M012195

TABLE 2.

Percentage of carbon formed from labeled substrate in medium

TG-Palmitate
TG-Glycerol
Substrate 5 mM Glucose 17.5 mM Glucose 5 mM Glucose 17.5 mM Glucose
% carbon % carbon % carbon % carbon
1 mM [1-13C]acetate 16.6 ± 1.7a ND ND ND
0.5 mM [U-13C]pyruvate 12.3 ± 2.3 12.3 ± 2.6 12.5 ± 1.1 13.9 ± 0.6
[U-13C]glucose 42.4 ± 2.0 47.0 ± 1.2b 72.1 ± 4.6 72.6 ± 5.1
2 mM [U-13C]glutamine 10.4 ± 0.7 9.2 ± 0.6 14.4 ± 0.5 13.4 ± 0.9

ND, not determined.

a

Because [1-13C]acetate was used, this is equivalent to 33.2% of TG-palmitate arising from medium acetate.

b

Significantly different from 5 mM [U-13C]glucose (P < 0.05).