Fig. 5.

In vivo measurements of energy expenditure (A, B) and ambulatory activity (C, D). Rats were placed into the CLAMS for 24 h after 4 and 8 weeks of treatment. Values from all animals were averaged with N=4 per group. Data were analyzed based on light and dark cycles. The first light cycle occurred from 11:00 to 19:00, where the animals were placed into the CLAMS immediately after their daily saline or AICAR injections. The dark cycle occurred from 19:00 to 07:00, and the second light cycle is from 07:00 to 10:00 in order to complete a full 24 h cycle in the metabolic cages. Two-way ANOVA with Bonferroni post hoc tests. *P < 0.05 versus control (Con) for each particular light/dark cycle. Data are expressed as mean ± SEM.