Fig. 6.

ICV insulin administration in diet-induced obese mice does not stimulate FA retention by WAT. Postabsorptive, body weight-matched WT mice received continuous ICV infusion of vehicle (white bars) or insulin (black bars, 0.5 mU/h) (A). Thirty minutes after starting the ICV infusion, the mice were infused for 2 h with glycerol tri[³H]oleate within VLDL-like emulsion particles and albumin-bound [¹⁴C]oleic acid. Subsequently, the mice were euthanized, and the retention of TG-derived FA and albumin-bound FA was determined in liver (B), heart (C), skeletal muscle (D), visceral fat (E), subcutaneous fat (F), and epigonadal fat (G). Values are means ± SEM for at least nine mice per group.