Fig. 3.

ApoE synthesis and turnover in adipocytes from apoE isoform knock-in mice. A: Cultured adipocytes were pulse-labeled with 200 μCi/ml ³⁵[S]methionine for 45 min and immediately harvested to measure incorporation of label into newly synthesized cellular apoE. B: Cultured adipocytes were pulse-labeled as described above followed by a 60 min chase incubation in DMEM supplemented with 500 μM unlabeled methionine. The amount of apoE secreted and retained in cells was directly measured, and the amount degraded was calculated as described in Materials and Methods. *P < 0.05, **P < 0.01 for comparison to apoE3.