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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Curr Opin Organ Transplant. 2011 Aug;16(4):345–352. doi: 10.1097/MOT.0b013e3283489a42

Figure 1. Role of chimeric cells of donor phenotype present in DBMC recipients at 1 year post-transplantation on MLR regulation.

Figure 1

Figure 1

(A & B): Recipient PMBC were used as responders in MLR either before (Total) or after the depletion of donor cells (D-Depleted) using monoclonal antibodies to mismatched HLA-Class I and Miltenyi magnetic microbeads. Statistically significant differences were obtained in the MLR responses to the donor between Total versus D-depleted recipient responders (p<0.01).

(C & D): 1×105 PMBC from renal transplant recipients depleted of donor chimeric cells were stimulated with 1×105 irradiated PMBC from the living related donors (C) or third Party (D) in presence of the indicated number of donor modulator cells and standard 3H-thymidine incorporation assays were performed on day 7. Data are shown as percentage inhibition ± SE (n=6). Statistically significant differences were obtained in the inhibition of anti-donor MLR between fresh DBMC versus RdD cells from the bone marrow (BM-RdD; p< 0.001) and fresh DBMC versus RdD cells from the peripheral blood (PBL-RdD; p<0.01).

[Previously published in Human Immunology: 2010; 71(6): 566–576]