| Component | Volume |
| Total RNA (up to 5 μg) | 10 μl |
| 10 mM dNTP mix | 1 μl |
| Oligo(dT)12-18 (0.5 μg/ μl) | 1 μl |
| DEPC-treated water | n μl |
| Total volume | 10 μl |
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