Table 1.

Two-dimensional gas chromatography/mass spectrometry (2D-GCMS) method parameters for analysis of cannabidiol (CBD), Δ⁹-tetrahydrocannabinol (THC), 11-hydroxy-Δ⁹-THC (11-OH-THC) and 11-nor-9-carboxy-THC (THCCOOH) in plasma

Front inlet		Back inlet
Mode	Constant pressure	Initial temp	100°C
Inlet temp	275°C	Initial time	10.40 min
Injection mode	Splitless	Ramp #1: 700°C/min	Final temp: 225°C
Purge flow	25.0 mL/min		Final time: 4.30min
Purge time	0.8 min	Ramp #2: 700°C/min	Final temp: 100°C
Pressure	35.0 psi		Final time: 5.50 min
Total flow	30.2 mL/min	Ramp #3: 700°C/min	Final temp: 275°C
			Final time: 0.00 min
Oven		Deans Switch
Initial oven temp	185°C	FID restrictor length	3.0 m
Initial oven hold	0.5 min	FID restrictor i.d.	0.10 mm
Ramp #1: 45°C/min	Final temp: 225°C	Aux 3 pressure	18.7 psi
	Final time: 3.00 min
Ramp #2: 15°C/min	Final temp: 275°C
	Final time: 1.58 min
Ramp #3: 80°C/min	Final temp: 195°C
	Final time: 2.30 min
Ramp #4: 10°C/min	Final temp: 230°C
	Final time: 0.00 min
Ramp #5: 25°C/min	Final temp: 275°C
	Final time: 5.10 min
Post-run		Flame ionization detector
Post temp	300°C	FID temp	250°C
Post time	1.0 min	Hydrogen flow	40.0 mL/min
Column 1 pressure	1.0 psi	Air flow	450 mL/min
Column 2 pressure	100.0 psi	Nitrogen makeup flow	45.0 mL/min