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. Author manuscript; available in PMC: 2012 Aug 5.
Published in final edited form as: Vaccine. 2011 Jul 13;29(34):5584–5590. doi: 10.1016/j.vaccine.2011.06.007

Fig. 1.

Fig. 1

Analysis of F1-V-MPR649-684 expressed in E. coli. (A) SDS-PAGE analysis of F1-V-MPR649-684 expression in E. coli. Lane 1, pre-induction control E. coli proteins; lane 2: total cell proteins 2 h post-induction; lane 3: soluble cell proteins after disruption of cells; and lane 4: F1-V-MPR649-684 purified by metal affinity chromatography. (B) Immunoblot analysis of E. coli-expressed F1-V and F1-V-MPR649-684. “Fusion” represents F1-V-MPR649-684. Polyclonal rabbit Abs to F1 (upper left) or V (upper right), or gp41-specific human mAbs 2F5 (bottom left) or 4E10 (bottom right) were used for detection. F1-V-MPR649-684 was detected by all Abs, whereas F1-V was recognized by anti-F1 and -V Abs only.