Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Mar 30;39(14):6100–6113. doi: 10.1093/nar/gkr182

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published by Oxford University Press 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Schematic representations of the tRNA-mediated suppression (TMS) systems, that use (A) monomeric and (B) dimeric tRNA reporter genes, the latter designated pDRT. The complementary read through (cRT) region in the monomeric construct is highly complementary to the sequence of the tRNA, so that if synthesized, it can fold back and interfere with processing of the tRNA (33). Note that for B, the upstream tRNA provides the pol III upstream promoter element (37). (C) Relative TMS activity of dimeric (pDRT) constructs bearing oligo(dT) tracts of varying length, T2-T7, in the T1 region, assayed in strain yAS99 as described (33) (actual activity of pDRT-4T was ∼75%). pJK148 is a negative control that contains no suppressor tRNA gene.