Figure 6.

Codon randomization leads to additional C11 termination readthrough mutants. (A) TMS spot assay comparing suppression activities of various individual position specific mutations of C11. See Table 3 for complete list. (B) TMS spot assay showing activities of mutants isolated by screening using the mini-library containing a mixture of all codon randomizations of 12 codons (see text). The identity key for the three columns is to the right. See Table 4 for complete list. (C) Pol II homology model with the three most frequent C11 mutants in red, each indicated by an asterisk, as reflected on the Rpb9p surface (yellow) as in Figure 5A.