Table 2.
Quantitation of terminator readthrough (relative to yAS68 cRT/U5)
Sample | dT(7); %RTa | dT(6); %RT | Relative increase in RT dT(7) to dT(6)b |
---|---|---|---|
yAS68-dT(3)c | 100 | 100 | – |
yAS99 | – | – | – |
+vector | 0 | <1 | – |
+Rpc2 | <1 | <1 | – |
+Rpc2 T455I | 7.4 | 21 | 2.8 |
+Rpc11 | <1 | <1 | – |
+Rpc11 F32S | 5.9 | 15 | 2.6 |
+Rpc11 C102S | <1 | <1 | – |
aReadthrough in yYH1; cRT signal/U5 signal, background corrected against +vector sample.
byAS77 {dT(6)} relative to yYH1 {dT(7)}.
ccRT/U5 signal of yAS68 is presumed to represent 100% readthrough.