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. 2011 Apr 12;39(14):6086–6099. doi: 10.1093/nar/gkr194

Figure 3.

Figure 3.

Regulation of HIF-1α translation by DDX6. (A) The DDX6 mRNA 3′UTR sites targeted by miR-130a (line) and -130b (dotted line) are shown. Mutagenesis of the DDX6 mRNA 3′UTR is indicated by arrows. (B) HEK293 cells were transfected with pre-miRNAs (25 nM) and either DDX6 3′UTR wild-type (left) or mutant (right) reporter genes, and then cells were cultured under normoxia. After 48 h, luciferase activities were analyzed and Renilla luciferase expression was normalized to firefly luciferase. Results represent means ± SD (n = 6, *P < 0.0001). (C) Endogenous DDX6 expression levels in the transfection of pre-miR-130a and -130b under normoxia were detected by western blots. Pre-miR N.C. was 100 nM. (D) Negative control and DDX6 siRNAs (50 nM) were transfected into HEK293 cells. After 8 h of hypoxia, HIF-1α expression levels were detected by western blots (left panel). The right panel shows HIF-1α expression in overexpression of DDX6.