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Trapping assays with Fpg mutants and other DNA glycosylases (a) The [cHyd:C]-trapping assays were carried out as previously described with no enzyme (−), the wild-type LlFpg enzyme (WT) and single-mutation P1G, ΔP1 and E2Q versions of LlFpg. (b) Similar comparative assays were performed by incubating the [cHyd:C] and [cHyd:A] DNA probes (as indicated) with no enzyme (lanes 1), Fpg (lanes 2), Nei (lanes 3) or hNEIL1 (lanes 4).
