Figure 6.
CD of ϕC31 Int and derivatives. Superimposed spectra of all the protein analysed are shown (panel A; near UV, panel B; far UV). The outliers in the near UV are hCTD (dotted line) and the wild-type Int (dashed line). All the remaining spectra in the near UV superimpose with the hInt protein. In the far UV the outliers are hCTD (crosses) and hIntS380D (circles); hInt and Int are the dashed and dotted lines, respectively. In the far UV spectra, all the hInt derivatives with an amino acid substitution therefore have slightly enhanced ellipticity compared with the hInt.