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. 2011 Apr 20;39(14):6223–6228. doi: 10.1093/nar/gkr250

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Self-cleavage of the RG ribozyme and mutants with a disrupted or restored kissing interaction. (A) Example time courses of RG self-cleavage at the indicated concentrations of MgCl₂. To display the wide range of observed cleavage rates, the time coordinate (X-axis) is plotted using a log₁₀ scale. (B) Apparent first-order cleavage rate constants (k_obs) for RNAs with an intact (RG, RGkI+V) or disrupted (RGkI, RGkV) kissing interaction. Data are the means of two to five replicates; SDs (data not shown for clarity) were typically less than the size of the symbols used to plot the means.