Table 1.

Results of in-house anti-HCV screening assay

	Mean	Minimum	Maximum	S.D.	CV%
Positive (n = 200)	0.82	0.58	0.91	0.051	6.21
Negative (n = 200)	0.17	0.09	0.18	0.012	6.66

Number of HCV positive and negative sera was 200 each. Cut-off value was calculated 0.21. Samples exhibiting O.D. greater than 0.21 were considered positive while those exhibiting less than 0.21 considered negative. Lower the value of Coefficient of Variation (CV%) more authentic the results. Statistically, CV% of maximum 10 is acceptable for the validation of the results. Coefficient of Variation percentage is mostly affected by the extreme values in the data.