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. 2011 Aug 8;6(8):e23349. doi: 10.1371/journal.pone.0023349

Figure 2. Effect of RNA helicase DHX36, DAZAP1, HuR and hnRNPA1 overexpression on ATM cryptic exon activation of pATMΔ and pΔSH5 minigenes.

Figure 2

(A) HeLa cells were transfected with pATMΔ and pΔSH5 minigenes in presence of empty expression vector (lanes 2 and 8, respectively) or vectors expressing the protein of interest (lanes 3-6 and 9-12, respectively). The splicing pattern was analyzed with the E16 and 2550 primers. RT-PCR fragments were resolved on 2% agarose gel. M is the molecular weight marker. On the right is indicated the splicing product with (+) or without (-) cryptic exon inclusion. The asterisk corresponds to a minor spliced product without the hybrid exon made of globin exon 3 and ATM exon 20. (B) The graph is the quantification of three independent experiments expressed as means ±SD.