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Differentiated Caco-2 cells were left untreated (medium) or were treated for 48 h with Nef, IFN-γ or their combination. Quantitative evaluation of apoptosis was performed by flow cytometry measuring co-staining of annexin V and propidium iodide. Means ± SEM from 5 independent experiments in duplicate, are shown. *p<0.05 vs medium; ○p<0.05 vs IFN-γ; Δp<0.05 vs Nef.
