Table 1.
Inhibition of soluble rhMMP-9 by alcohols
| Chemical | Concentration | % Inhibition | IC50 (moles/L) |
|---|---|---|---|
| methanol | 4.28 M | 12.4 ± 1.57%A | 19.1 ± 2.86%a |
| ethanol | 4.28 M | 14.3 ± 2.06%A | 19.6 ± 0.36%a |
| 1-propanol | 4.28 M | 79.7 ± 0.18%F | 2.7 ± 0.13%d |
| 2-propanol | 4.28 M | 52.7 ± 0.18%D | 4.4 ± 0.13%e |
| 1-butanol | 1.71 M* | 56.1 ± 0.09%D | 1.6 ± 0.09%c |
| 2-butanol | 4.28 M | 78.7 ± 0.94%F | 0.5 ± 0.04%b |
| tert-butanol | 4.28 M | 91.1 ± 0.18%G | 2.7 ± 0.04%d |
| 1-pentanol | 0.86 M* | 28.5 ± 0.54%B | 1.7 ± 0.04%c |
| 2-HEMA | 4.28 M* | 99.4 ± 0.27%H | 0.1 ± 0.09%b |
| 1-hexanol | 0.17 M* | 28.3 ± 0.40%B | -- |
| 2-hexanol | 0.17 M* | 13.2 ± 0.67%A | 1.6 ± 0.09%c |
| 3-hexanol | 0.17 M* | 35.1 ± 1.16%E | 0.4 ± 0.04%b |
| 1-heptanol | -- | -- | -- |
| 2-heptanol | 0.17 M* | 13.7 ± 2.33%A | 3.0 ± 0.18%d |
| 3-heptanol | 0.17 M* | 17.1 ± 0.54%A | 0.5 ± 0.04%b |
| 1-octanol | 0.17 M* | 25.4 ± 1.21%B | 0.3 ± 0.22%b |
| 2-octanol | 0.17 M* | 21.5 ± 0.63%B,C | 2.7 ± 0.18%d |
| 3-octanol | 0.17 M* | 10.1 ± 1.79%A | -- |
| 4-octanol | 0.17 M* | 17.7 ± 0.27%A,C | 1.1 ± 0.04%c |
| 1,2-ethanediol | 4.28 M | 36.6 ± 0.18%E | 5.8 ± 0.36%e |
| 1,3-propanediol | 4.28 M | 84.4 ± 0.60%I | 1.1 ± 0.27%c |
Groups identified by different uppercase and lowercase superscript letters are significantly different (p±0.05). Values are mean ± SEM (n=5) as determined by least squares means method.
Higher molecular weight alcohols had lower solubilities than the lower molecular weight alcohols. The concentrations indicated are their highest soluble concentrations in the proprietary assay buffer. Alcohols highlighted in grey, inhibited rhMMP-9 by more than 50%. Their inhibitory activity was also assayed at lower concentrations of 1.71, 0.86 and 0.17 moles/L to obtain a dose-response relationship. That data is not shown but was used to obtain the IC50 values by plotting percent rhMMP-9 inhibition vs. alcohol concentrations.