Table 1.
Summary of targeting experiments using TALENs OCT 4
| correctly targeted clones | ||||||||
|---|---|---|---|---|---|---|---|---|
| cell line targeted |
TALEN pair |
donor | # of clones picked |
random integration |
targeted + additional integration |
heterozygous | homozygous | targeting efficiency [%] |
| WIBR#3 hESC | OCT4 intron1 | OCT4-SAeGFP# 1 | 68 | 6 | 0 | 62 | 0 | 91 |
| C1 iPSC | OCT4 intron1 | OCT4-SAeGFP# 1 | 7 | 0 | 0 | 7 | 0 | 100 |
| WIBR#3 hESC | OCT4 intron1 | OCT4-SAeGFP# 3 | 35 | 0 | 0 | 34 | 0 | 97 |
| C1 iPSC | OCT4 intron1 | OCT4-SAeGFP# 3 | 5 | 0 | 0 | 5 | 0 | 100 |
| WIBR#3 hESC | OCT4 intron1 | OCT4-eGFP exon1 fusion | 12 | 4 | 0 | 8 | 0 | 67 |
| C1 iPSC | OCT4 intron1 | OCT4-eGFP exon1 fusion | 1 | 0 | 0 | 1 | 0 | 100 |
| WIBR#3 hESC | OCT4 STOP | OCT4-eGFP PGK-Puro (C-term fusion) | 48 | 42 | 1 | 5 | 0 | 10 |
| C1 iPSC | OCT4 STOP | OCT4-eGFP PGK-Puro (C-term fusion) | 48 | 45 | 3 | 0 | 0 | 0 |
| WIBR#3 hESC | OCT4 STOP | OCT4-2A-eGFP PGK-Puro (C-term fusion) | 48 | 44 | 1 | 3 | 0 | 6 |
| C1 iPSC | OCT4 STOP | OCT4-2Ae-GFP PGK-Puro (C-term fusion) | 48 | 46 | 1 | 1 | 0 | 2 |
| WIBR#3 hESC | OCT4 STOP | OCT4-eGFP-2A-Puro (genetrap) | 48 | 0 | 26 | 22 | 0 | 46 |
| PPP1R12C | ||||||||
| WIBR#3 hESC | PPP1R12C | PPP1R12C-SA-2A-Puro | 96 | 4 | 35 | 34 | 21 | 57 |
| WIBR#3 hESC | PPP1R12C | PPP1R12C-PGK-Puro | 107 | 19 | 23 | 52 | 13 | 61 |
| WIBR#3 hESC | PPP1R12C | PPP1R12C-CAGGS-eGFP | 32 | 1 | 16 | 12 | 3 | 47 |
| C1 iPSC | PPP1R12C | PPP1R12C-SA-2A-Puro | 24 | 1 | 7 | 8 | 8 | 66 |
| C1 iPSC | PPP1R12C | PPP1R12C-PGK-Puro | 55 | 4 | 24 | 19 | 8 | 49 |
| C1 iPSC | PPP1R12C | PPP1R12C-CAGGS-eGFP | 23 | 1 | 12 | 5 | 5 | 43 |
| WIBR#3 hESC | PPP1R12C eHiFi | PPP1R12C-SA-2A-Puro | 22 | 4 | 5 | 14 | 3 | 77 |
| C1 iPSC | PPP1R12C eHiFi | PPP1R12C-SA-2A-Puro | 20 | 0 | 7 | 9 | 4 | 65 |
| PITX3 | ||||||||
| WIBR#3 hESC | PITX3 Exon 1 | PITX3 eGFP FW | 96 | 62 | 22 | 11 | 1 | 13 |
| C1 iPSC | PITX3 Exon 1 | PITX3 eGFP FW | 96 | 88 | 4 | 4 | 0 | 4 |
| C1 iPSC | PITX3 Exon 1 | PITX3 eGFP BW | 96 | 84 | 11 | 1 | 0 | 1 |
| WIBR#3 hESC | PITX3 STOP | PITX3-eGFP C-term fusion | 48 | 20 | 16 | 11 | 1 | 23 |
| C1 iPSC | PITX3 STOP | PITX3-eGFP C-term fusion | 48 | 32 | 7 | 9 | 0 | 19 |
| WIBR#3 hESC | PITX3 STOP | PITX3-eGFP C-term 2A fusion | 96 | 32 | 44 | 19 | 1 | 21 |
| C1 iPSC | PITX3 STOP | PITX3-eGFP C-term 2A fusion | 48 | 24 | 13 | 9 | 2 | 23 |