A–F: JNK is phosphorylated in the soma and nucleus of a SGN in vitro, but not in adjacent glia, six hours after transfer to medium lacking neurotrophic support (5K, A–C) from depolarizing (25K, D–F) medium. Scale bar = 10 μm. A,D: NF200 (green) and bisbenzamide nuclear label (blue). B,E: pJNK immunofluorescence (red). C,F: merged NF200, nuclear and pJNK labeling. G–L: JNK is phosphorylated in a punctate pattern in the growth cone 30 minutes after transfer to medium lacking neurotrophic support (5K, G–I) from depolarizing (25K, J–L) medium. Scale bar = 5 μm. G,J: Lck-GFP (green). H,K: pJNK immunofluorescence (red). I,L: merged Lck-GFP and pJNK labeling.