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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Hear Res. 2011 Apr 22;278(1-2):86–95. doi: 10.1016/j.heares.2011.04.005

Fig. 4. Wnt-expressing HEK cells do not affect HH20-25 SAG neurite outgrowth in a co-culture assay.

Fig. 4

Confocal images of SAG explants co-cultured for 40h with control (Untransfected) or Wnt-transfected HEK cells (A-G). Lines indicate the edge of HEK cell aggregates (rightmost in each image). Explants cultured with control and Wnt-transfected cells display similar neurite outgrowth patterns. Scale bar=200 μm. Quantification of neurite length (H) and pixel number (I) for quadrants facing the cell aggregate. Each bar represents the mean (± SE) computed from SAG explants within a treatment group. Sample numbers for each treatment are above the bars. There is no significant difference in average neurite length (H; p=0.6142) or pixel number (I; p=0.1112) between explants grown with control or Wnt-transfected cells.