Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Nat Biotechnol. 2011 Jul 17;29(8):757–761. doi: 10.1038/nbt.1918

Figure 2. Expression of iRFP in living mouse.

Figure 2

(a) Overlay of representative light and fluorescent images of iRFP or IFP1.4 adenovirus infected mice with and without injection of 250 nmol BV. A non-infected control mouse is shown on the right. The fluorescence images were acquired using IVIS Spectrum instrument equipped with 675/30 nm excitation and 720/20 nm emission filters. The color bar indicates the fluorescence radiant efficiency, multiplied by 109. (b) Near infra-red fluorescence total radiant efficiency of the liver areas of the iRFP and IFP1.4 expressing mice in (a), normalized to the bandwidth of the excitation and emission filters. (c) Time course of the NIR fluorescence total radiant efficiency of the liver areas of the iRFP and IFP1.4 expressing mice in (a) after BV injection. (d) Overlay of the photograph and fluorescent image of the isolated livers from the BV-injected infected and non-infected (control) mice. (e) Time course of the NIR fluorescence total radiant efficiency of the liver areas of the mice not being injected with BV. The fluorescence signals were normalized to the bandwidth of the excitation and emission filters.