Table 1.
Primer List
| Primer | Sequence | Amplicon |
|---|---|---|
| PKH051981.-1449For | GGAGAAGGAATGTGGATAACGAATG | 5'UTR |
| PKH051981.46Rev | TCTTCTTCATCCATTCTTCCAACAA | |
| PKH051981.27For | GGAAGAATGGATGAAGAAGACTG | Exon 1-exon2 of PKH_051981 |
| PKH051981.946Rev | ATATCGTCTCTAAGGTCTGCATTAG | |
| PKH051981.946RevT7 | TAATACGACTCACTATAGGGAGGATATCGTCTCTAAGGTCTGCATTAG | |
| PKH051981.482For | ACATCCATCGAACCACATGAA | |
| PKH051981.985Rev | GGTGTAACTGCCGTCAGTTAA | |
| 205A/B EXON1.66For | CACTTCAAATCTGACGACACTAATG | Revised exons 2–4 across gap |
| 205A/B EXON1.62For | TGGACACTTCAAATCTGACGAC | |
| 205A/B EXON2.653Rev | GATGTTCCAGGTTTAGTCCCC | |
| 205A/B EXON2.390Rev | GCCACTTCCTCCTATCTTAGCAC | |
| 205A/B EXON1.222Rev | CGCTGCTGCTTCATCTCTTG | |
| PKH051981.849For | GATTTTTGCGGAGAGGACTG | Non-coding region downstream of exon 2 |
| PKH051981.838For | GAATAGGAATGAGGAAGAAGCC | |
| PKH051981.526For | GGCTGTCGCATTGAACGAAC | |
| PKH051981.2874Rev | CCTAAGCAACCTTCTTTCCTTG | |
| PKH051981.1681Rev | TCCCTTCCATTCCTTCCCTCTC | |
| PKN385d08.45For | CCTTCTTTCCATCATTCTTTCATCC | Non-coding region upstream of PkH_000630 |
| PKN385d08.260Rev | CGCTGCTGCTTCATCTCTTGTC | |
| PKH051981.526For | GGCTGTCGCATTGAACGAAC | Non-coding region downstream of PKH_051981 |
| PKH051981.838For | GAATAGGAATGAGGAAGAAGCC | |
| PKH051981.849For | GATTTTTGCGGAGAGGACTG | |
| PKH051981.1681Rev | TCCCTTCCATTCCTTCCCTCTC | |
| PKH051981.2874Rev | CCTAAGCAACCTTCTTTCCTTG | |
| 205exon11gsp7.Rev | GTTCAACTTCTTTCTGCTTATTTCCC | Exon 11 oligoprobe |
| 205exon2.1F | CAGCAATGACGAAGGATGGAATC | Exon 4 probe |
| 205A/B EXON2.653Rev | GATGTTCCAGGTTTAGTCCCC | |