Figure 2.
TNF-α primed response of SW982 synoviocytes to noxious thermal challenge. Effect of TNF-α pre-treatment on [Ca2+]i response to noxious (45°C) thermal challenge in SW982 synoviocytes. A and B: representative calcium traces recorded from (A) control (vehicle treated) synoviocytes and (B) TNF-α (1 ng/ml, 8 hr) pre-treated cells. (C) Peak and average calcium response in control (white bars, n = 7), or TNF-α (1 ng/ml) treated synoviocytes. The TNF-α was added to the incubation media for 4 hr (n = 3 trials), 8 hr (n = 4 trials), 12 hr (n = 3 trials), or 16 hr (n = 3 trials) before the experiment.* indicates a statistically significant difference (p < 0.05) assessed by ANOVA with Dunnett's test.