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. 2011 Apr 26;8:192. doi: 10.1186/1743-422X-8-192

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Copyright ©2011 Zaremba et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Spry1 interacts with E1A_13Sof the non-oncogenic Adenovirus type 2 (Ad2) and with the Human papillomavirus type 16 (HPV16) E7 protein. Lysates from cell extracts expressing Spry1 were incubated with (A) GST-Ad2-E1A_13Sand (B) GST-HPV16-E7-fusion proteins or for control reactions with the leader sequence GST or GST-Ad12-E1A_13Sas indicated. The assays were performed with 0.5 mg of cellular lysate and 40 μg of GST-fusion proteins at 4°C for 1 h. The bound proteins were subjected to SDS-PAGE and analyzed by immunoblotting. Spry1 was detected by an antibody directed against the Myc-epitope of the Spry-fusion protein.