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. 2011 Apr 26;8:192. doi: 10.1186/1743-422X-8-192

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Copyright ©2011 Zaremba et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Spry1 co-localizes with E1A_13sin the cytoplasm. The subcellular localization of Spry1 and E1A_13swas determined in HeLa cells by confocal immunofluorescence microscopy using antibodies that specifically recognize Spry1 (green) and the Myc-tagged E1A_13s(red) protein constructs. (A) Spry1 and (B) E1A_13stransfected cells were left serum starved overnight and stimulated with 20 ng/ml bFGF for 2 h. (C) Images were overlaid for comparison of staining, yellow indicates co-localization of the two proteins. Scale bar, 10 μm.