Figure 7.

Concentration-dependent suppression of Spry1 function by E1A_13S. HeLa cells were co-transfected with the SRE-Luciferase reporter construct and different concentrations of expression plasmids coding for Spry1 and/or E1A_13S. We used either constant amounts of Spry1- (A) or E1A_13S-expression vectors (B) in our experiments as indicated. Empty expression vectors were added to keep the amount of transfected DNA constant. After transfection cells were left serum-deprived for 24 h and followed by incubation with 20 ng/ml bFGF in DMEM. The promotor activity of the reporter gene in the presence of empty vectors was set as 1.