Table 1.
Primers for PCR amplification of the human TWSG1 gene, coding exons 2-5
| Exon | Forward | Reverse | Product size (bp) |
Tm* (°C) |
|---|---|---|---|---|
| 2 | 5′ CTG GGA GTT ACT GAT CAT CTT CTT 3′ |
5′ ATA AAC ATA ATG TTT CTG CTC TAC 3′ |
219 | 52 |
| 3 | 5′ ATT TGG AAT TTG AAG TTT AAC ATC 3′ |
5′ AGA AAT ATT AAG AAG TCT CCC TTG 3′ |
174 | 53 |
| 4 | 5′ CTA ACA AAA TCT TAT GAT ATT ACC 3′ |
5′ GGC GGA ATG GAA AAA GTC AAC T 3′ |
335 | 52 |
| 5 | 5′ TGC CCT GAA ATC TTA AAT TTT TGT 3′ |
5′ AAG TTG CTT TGG TTT GCA TTT GTC 3′ |
241 | 53 |
*
Annealing temperature for PCR amplification