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The 120FN inhibits mineralized nodule formation in PDL cells. PDL cells were cultured in osteogenic/mineralizing FN-free serum medium without or with 120FN (0.1 μM) for 14 days. The cells and matrix were subjected to von Kossa staining (A). For quantification, mineralized nodules were counted for a total of six different fields per well (B). Data are shown as mean (+ SD) fold change relative to control baseline levels for three experiments performed in triplicate (***, p < 0.001).
