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Sensitivity of the TaqMan real-time PCR assay for Pbo-likeV detection. Ten-fold dilutions of the standard template pMD18-NP1 containing the target nucleotide sequence were amplified using the real-time PCR assay. Amplification plots of 2.00 × 107 to 2.00 × 101 copies/μL of pMD18-NP1 were detected by real-time PCR assay.
