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Primers and probe used in real-time PCR assay for Pbo-likeV
| Gene | Primer-probe | Sequence | Positiona |
|---|---|---|---|
| NP1 | Forward | 5'-TCGAGCTATACAACCGAAGAAGAGA-3' | 37-61 |
| Reverse | 5'-TGTTTCGGAGATGTCCTTGCT-3' | 93-113 | |
| Probe | FAM-5'-CAGCTCTTCGAATCGCCGCTCTCC-3'-TAMRA | 63-86 |
aNucleotide position was designated according to NP1 gene of Pbo-likeV strain JSNJ1 (GenBank accession no. HQ872052)
