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. 2010 Oct 4;1:121. doi: 10.3389/fphar.2010.00121

Figure 2.

Figure 2

Long-range (XL) PCR products generated from hybrid genes and control samples. Fragment designation corresponds to that provided in Figure 1 and used throughout this manuscript. The genotypes of the cases are shown in Table 1. PCR product lengths are given in kilobyte to the right, marker sizes on the left. All PCRs were carried out on genomic DNA. (A) Triplex XL-PCR showing the formation of fragment A (entire CYP2D6 gene), fragment B (presence of gene duplication), and fragment H (indicating the presence of a CYP2D7/2D6 hybrid gene). (B) Fragment H was generated by itself. Note that this fragment is weak in the triplex reaction in panel A for case 9, but unequivocally present when amplified “solo”. (C) As expected from sequence data, cases 5, 6, and 7 generated a PCR product spanning CYP2D8 to CYP2D6 intron 6. CYP2D6*66 (index case) and case 9 serve as negative controls (see Figure 3 for hybrid details).