Table 1.
List of strains and plasmids.
| Strain/plasmid | Relevant characteristic (s) | Source or reference |
|---|---|---|
| B. ABORTUS STRAINS | ||
| S2308 | B. abortus wild-type isolate, type strain (biovar 1) | NADC, bovine passaged |
| S2308ΔmanBA | S2308 ΔmanBA::KanR | This study |
| B. MELITENSIS STRAINS | ||
| 16M | B. melitensis wild-type isolate, type strain (biovar 1) | ATCC, goat passaged |
| 16MΔmanBA | 16M ΔmanBA::KanR | This study |
| E. COLI STRAINS | ||
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ- | Invitrogen |
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| DH5α | F− Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK−, mK+) phoA supE44 λ− thi-1 gyrA96 relA1 | Invitrogen |
| ß2155 | thrB1004 pro thi strA hsdS lacZ ΔM15 (F9 lacZ ΔM15 lacIq traD36 proA1 proB1) ΔdapA::erm (ErmR) pir::RP4 (::kan (KanR) from SM10) | Dehio and Meyer (1997) |
| PLASMIDS | ||
| pBBR1MCS6-y | Cloning vector, CmR, GFP | Murphy et al. (2002) |
| pBluescript KS II (+) | Cloning vector | Stratagene |
| pKD4 | Source of KanR cassette and FRT sites | Datsenko and Wanner (2000) |
| pJET1445 | B. melitensis manBA operon in pBBR1MCS6-y, CmR | This study |
| pJET931MA | pBluescript containing manBA 3′ and 5′ fragments, used to construct pJET940MA, AmpR | This study |
| pJET940MA | Suicide vector for deletion of manBA, KanR, AmpR | This study |