Table 2.

Primers used in this study.

Primer	Sequence (engineered restriction site)	Purpose
TAF204	5′-GGCGCGCCACGTCTTGAGCGATTGTGTAGG-3′ (AscI)	Kan cassette
TAF205	5′-GGCGCGCCGGACAACAAGCCAGGGATGTAAC-3′ (AscI)	Kan cassette
TAF251	5′-CGGGATCCCGGCAAAGGGTTCCGCAATAC-3′ (BamHI)	5′ manBA fragment
TAF254	5′-GGCAAAATACCGGCGCGCCGCAAATCCCTGCCGACAAAC-3′ (AscI)	5′ manBA fragment
TAF252	5′-CGGGATCCCGGGTCAGTCATCAGTTGCGGATTC-3′ (AscI)	3′ manBA fragment
TAF253	5′-GCAGGGATTTGCGGCGCGCCGGTATTTTGCCCCTCGTCCTG-3′(AscI)	3′ manBA fragment
TAF419	5′-TTCCAGGCAGATACAGG-3′	manBA amplification
TAF420	5′-ATCCCAATAGGCCGAATGCCAA-3′	manBA amplification
TAF433	5′-GAAGATCTTCCAGGCAGATACAGG-3′ (BglII)	manBA amplification
TAF434	5′-GAGATCTATCCCAATAGGCCGAATGCCAA-3′ (BglII)	manBA amplification
TAF234	5′-GCTCTCCATGATTTCGGGTA-3′	nylC amplification
TAF235	5′-CGCTTCGCAAAATTGAAAAT-3′	nylC amplification