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. 2011 Apr 18;2:79. doi: 10.3389/fmicb.2011.00079

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Copyright © 2011 Beard, Paradela, Albar and Jerez.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Extracellular A. ferrooxidans deposits contain elemental sulfur. (A) Time course of elemental sulfur production by A. ferrooxidans cultures subjected to OSC- (▼) or OLC- (∇) incubations. Aliquots of A. ferrooxidans cultures were taken at the times indicated and cells and sulfur were sedimented by centrifugation. Sulfur was extracted from the pellets with acetone and determined by cyanolysis. (B) Phase-contrast micrographies of A. ferrooxidans cells (600 × magnification) after 10 h OLC- or OSC-incubation. Panels labeled AT correspond to cells obtained after resuspending acetone-treated pellets in DSMZ 71 medium (without thiosulfate).