Figure 2.

Characterization C. burnetii Nine Mile (phase II) MC1 and B2c Himar1 transformants. (A) Schematic of the Himar1 chromosomal integration site in C. burnetii MC1. The Himar1 transposon is flanked by inverted terminal repeat (ITR) elements and inserted into an intergenic region between CBU0316 and CBU0317. Flow cytometry (B) and confocal fluorescence microscopy (C) of live Vero cells infected with MC1 or B2c for 5 days. Both assays revealed considerably more mCherry fluorescence from MC1-containing vacuoles, where expression is driven by 311^P, then from B2c-containing vacuoles, where expression is driven by 1169^P (Beare et al., 2009). The green trace in flow cytometry histograms shows autofluorescence of Vero cells infected with wild-type C. burnetii for 5 days. Bars, 5 μm.