Figure 4.

Coxiella burnetii Tn7 transformation system. (A) Maps of two-plasmid C. burnetii miniTn7 transposon system. The suicide plasmid pTNS2::1169^P-tnsABCD encodes the tnsABCD operon under control of 1169^P. The suicide plasmid pMiniTn7T-CAT::GFP encodes CAT and GFP genes driven independently by 1169^P and 311^P, respectively. The CAT/GFP cassette is flanked by Tn7L and Tn7R elements. (B) Schematic of the glmS regions in C. burnetii Nine Mile (phase II) and C. burnetii Nine Mile (phase II)/Tn7-CAT-GFP. (C) Fluorescence microscopy of Vero cells infected for 5 days with C. burnetii Nine Mile (phase II)/Tn7-CAT-GFP (green). Cells were fixed with 4% paraformaldehyde, then immunostained for the lysosomal protein CD63 (red). Bar, 5 μm.