. Author manuscript; available in PMC: 2011 Aug 9.

Published in final edited form as: Arch Biochem Biophys. 2009 Aug 15;488(2):100–104. doi: 10.1016/j.abb.2009.07.004

Table 2. Activities of ME1 and ME2 in INS-1 832/13 cells.

Enzyme activities were measured and yields of mitochondria were calculated as described under Experimental Procedures.

Specific Enzyme Activity of Malic Enzymes Based on Cytosol or Mitochondria Protein
ME1 in cytosol	29.1 ± 1.6 nmol NADPH/min/mg cytosol protein^a
ME2 in mitochondria	20.5 ± 0.4 nmol NADPH/min/mg mitochondrial protein^b

	Specific Enzyme Activities Based on Cell Protein (nmol NADPH/min/mg total cell protein)	% of Total
ME1 + ME2 (Measured in homogenate)	18.7 ± 0.9	100%
ME1 (Calculated from measurement in cytosol)^a	8.7 ± 0.4	47%
ME1 (Calculated from measurement in whole cell homogenate)^a	12 ± 0.1	64%
ME2 (Calculated from activity in mitochondria and yield of mitochondria)^b	6.6 ± 0.08	35%
ME2 (Calculated by subtracting ME1 activity^a from total ME activity^b in homogenate)	6.7 ± 0.9	36%

Activity measured in presence of 1 mM malate

Activity measured in presence of 10 mM malate plus 5 mM fumarate.