. Author manuscript; available in PMC: 2011 Aug 9.

Published in final edited form as: Arch Biochem Biophys. 2009 Aug 15;488(2):100–104. doi: 10.1016/j.abb.2009.07.004

Table 3. Mitochondrial NAD(P) malic enzyme ME2 activity is present in INS-1 832/13 cells and pancreatic islets of the mouse, rat and human.

Mice were the ICR and NSA strains. ME2 activity was measured as described under Experimental Procedures without or with ATP (2 mM). Results are the mean with the number of preparations of enzyme source in parentheses and SE (when N ≥ 3).

Enzyme Source	ME2 Specific Enzyme Activity (nmol NADPH/min/mg protein)
INS-1 832/13 homogenate + ATP	6.7 ± 0.9 (3)^a 2.3 ± 0.2 (3)^a
INS-1 832/13 mitochondria + ATP	25 ± 3.9 (5) 4.8 ± 2.6 (3)
Mouse islet whole cell homogenate + ATP	18 ± 3.6 (3) 5.1 ± 0.9 (3)
Rat islet whole cell homogenate + ATP	5.4 ± 1.1 (5) 0 (2)
Rat islet mitochondria + ATP	12 (1) 0 (1)
Human islet whole cell homogenate + ATP	4.7 ± 0.22 (7) 0.8 ± 0.5 (7)
Human islet mitochondria	18 ± 3.6 (3)

Rate is extrapolated to basis of whole cell protein even though measured in mitochondrial fraction.