Skip to main content
. 2010 Feb 1;25(6):1282–1294. doi: 10.1002/jbmr.4

Fig. 4.

Fig. 4

IKKβSSEE induction of osteoclasts does not require IKKα. (A) Wild-type and IKKα null FLCs expressing GFP were cultured in M-CSF with or without RANKL or IKKβSSEE with M-CSF alone. TRACP stain was used for osteoclasts, phalloidin stain for actin rings, and toluidine (Tol.) blue stain for dentin resorption. Wild-type cells infected with IKKβSSEE generated 133 ± 22 osteoclasts per well compared with 112 ± 12 osteoclasts per well in IKKβ1 null cells infected with IKKβSSEE. Percent resorbed area was 22% ± 5& and 21& ± 7%, respectively. Scale bars indicate magnification of actin image. (B) Western blot for expression of NF-κB pathway markers. OC+ = osteoclast-positive control; n.s. = nonspecific band. (C) Relative expression of mRNA for markers of osteoclastogenesis by real-time qPCR. Levels normalized to GAPDH.