Fig. 6.

Active IKKβ is sufficient for osteolysis. (A) Images of TRACP-stained histologic slides of calvarial bones of mice injected supracalvarially with adenovirus expressing LacZ (Ad LacZ) or IKKβ^SSEE (Ad IKKβ^SSEE), LPS, or RANKL. Arrows indicate areas of osteoclastic bone erosion. Scale bars = 200 µm. (B) Images of H&E-, TRACP-, and immunoperoxidase-phospho-IκBα−stained histologic slides of knees of mice injected intraarticularly with adenovirus expressing LacZ (Ad LacZ) or IKKβ^SSEE (Ad IKKβ^SSEE). Arrows indicate areas of pathologic osteoclastic bone erosion (pink) at the articular surface. Asterisks denote areas of potential inflammatory cell infiltrate into the synovial space. Arrowheads in TRACP-stained sections point to remnants of articular cartilage. Arrowheads in lower panel show positive stain for phosphorylated IκBα. Objective used for capturing image is labeled above panels. Scale bars = 200 µm.