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Optimization of MgCl2 concentration and the annealing temperature to detect Salmonella spp., Salmonella Typhi and Salmonella Typhimurium. Lane M shows a 100-bp DNA ladder. Lanes 1 to 3 show the PCR products with 2.0 mM MgCl2 and lanes 4 to 6 with 2.5 mM MgCl2. The annealing temperature was 50°C for lanes 1 and 4; 53°C for lanes 2 and 5; and 56°C for lanes 3 and 6.
