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Primers for RT-LAMP amplification of the HA gene of H5N1 influenza viruses. K, R, W, Y is the mixture of (G, T), (A, G), (A, T) and (C, T) respectively.
| Primers | Sequence | Gene position (HN 30850) |
|---|---|---|
| H5-F3 | GCTATAGCAGGKTTTATAGAGG | 1048-1069 |
| H5-B3 | GCCTCAAACTGAGTGTTCAT | 1210-1229 |
| H5-FL | GGTGRWACCCATACCAACCA | 1092-1111 |
| H5-BL | CYACTCAAAAGGCAATAGATGGA | 1154-1176 |
| H5-FIP | ACTCCCCTGCTCRTTGCTATGGATGGCAGGGAATGGTA | 1074-1089 |
| 1110-1131 | ||
| H5-BIP | GGTACGCTGCAGACAARGAATGAGTTGACCTTATTGGTGA | 1133-1153 |
| 1178-1196 |
