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. 2010 Dec 14;2(12):2824–2836. doi: 10.3390/toxins2122824

Figure 1.

Figure 1

Effect of glycophorin and LtxA on RBCs. (a) RBCs were treated with water (100% lysis), LtxA buffer (background lysis), LtxA, or first pre-treated with anti-glycophorin antibody (ab) and then LtxA. Lysis was measured by detecting released hemoglobin in the supernatant at 450 nm. The data shown is representative of three independent experiments. (b) Flow cytometry of RBCs stained with anti-glycophorin antibody alone or after pre-treatment with LtxA. The shift in signal to the right represents cells that are stained with anti-glycophorin antibody. The data shown is representative of three independent experiments.