Figure 6.

Average metabolic responses of PC-12 cells exposed to either 100 nM CTx or 500 nM CTxB subunit. Cell necrosis is triggered at 300 minutes. (a) Lactate production. The average of four chambers () treated with three 2-minute pulse of 500 nM CTxB (◊). The other trace is the average of four chambers () treated with three 2-minute pulse of 100 nM CTx (♦). (b) Oxygen Consumption. The average of four chambers () treated with three 2-minute pulse of 500 nM CTxB (◊). The other trace is the average of three chambers () treated with three 2-minute pulse of 100 nM CTx (■). (c) Acid production. The average of four chambers () treated with three 2-minute pulse of 500 nM CTxB (◊). The other trace is the average of four chambers () treated with three 2-minute pulse of 100 nM CTx (■).