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. 2010 Apr 8;2(4):632–648. doi: 10.3390/toxins2040632

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© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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The metabolic response of PC-12 cells pre-treated with 1 µg/mL BrA. Necrosis is triggered at 330 minutes. (a) Lactate production. One chamber () treated with brefeldin A (black bar), and then exposed to ten minutes of 10 nM CTx (♦). The lactate sensor in the replicate chamber failed to calibrate, so N = 1. The other chamber () received only CTx. (b) Acid production. The average of two chambers () treated with brefeldin A (black bar), and then exposed to ten minutes of 10 nM CTx (♦). The other chamber () received only CTx.