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. 2010 May 28;2(6):1179–1206. doi: 10.3390/toxins2061179

Figure 2.

Figure 2

Proposed mechanism of SPATEs autoproteolytic processing. (A). EspP was proposed to be cleaved by an autoproteolytic mechanism taking place in the pore of the autotransporter β-barrel. (B). After translocation of the passenger domain across the OM, Asn1023 is positioned close to Asp1120. As a consequence of hydrogen bonding between the two side chains, Asn1023 amide group performs a nucleophilic attack on the Asn1023–Asn1024 peptide bond. This reaction results in Asn1023 cyclization and the production of a succinimide intermediate at the C-terminus of the passenger domain, which will be ultimately hydrolyzed into a mixture of asparagine and iso-asparagine [64,65].