Table 2.

SPATEs substrate specificity. Listed are the substrates for which SPATE activity has been tested.

	Cleaved	Not cleaved
EatA	AAPM-pNA, AAPL-pNA [25]
EpeA	pepsin A, gelatin, mucin [16]
EspC	fodrin [71], hemoglobin [72], pepsin, factor V, spectrin (fodrin) [20,40]	Human IgA1 [71], mucin [40], lysozyme [73]
EspI	pepsin A, apolipoprotein A1 [26]	IgA1, hemoglobin, bovine serum albumin, α2-macroglobulin, haptoglobin, thrombin, collagene type 3, trypsin, high density lipoprotein, low density lipoprotein, very low density lipoprotein, trypsin, transferrin, lactoferrin, pepsinogen, gelatin, casein [26]
EspP	pepsin A, human coagulation factor V [14], casein [15], apolipoprotein A1 [26], AAPL-pNA [40]	human IgA1, bovine serum albumin, α2-macroglobulin, transferrin, lactoferrin, pepsinogen [14], mucin, spectrin (fodrin) [40]
Hbp	hemoglobin [5]	albumin, human lactoferrin, human immunoglobulin A1 [5]
Pet	casein, gelatin [17], pepsin, human coagulation factor V, spectrin [19,40]	actin [75], mucin [19,40]
Pic	gelatin, ovomucin, bovine mucin, murine mucin [18], human spectrin (fodrin), pepsin A, human coagulation factor V [19,40], mucin [19]	casein, IgA, IgM, IgG, hog gastric mucin [18] ovine spectrin [40]
Sat	casein [22], factor V, spectrin [40]	IgA1 [22], hemoglobin, mucin, pepsin [40]
SepA	FLF-pNA, VPF-pNA, AAPF-pNA, AAPM-pNA [70]	IgA1, gelatin [28], angiotensin-I, egg lysozyme [70], fibronectin, mucin, pepsin, factor V, spectrin (fodrin) [40]
SigA	casein [29], fodrin [45]
Tsh	mucin, factor V [40]	human and chicken IgA, casein, pepsin A [40,42], spectrin [40]
Vat		casein [35]