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. 2010 Jul 19;2(7):1881–1897. doi: 10.3390/toxins2071881

Figure 2.

Figure 2

cAMP production in ET-treated murine naïve B lymphocytes. 5 × 105 naïve B lymphocytes in 200 μL were plated in each well of a 96 well tissue culture plate and treated with ET (2.5 μg/mL PA and 1.0 μg/mL EF) and intracellular cAMP was measured using a direct cAMP Enzyme Immunoassay kit according to manufacturer’s instructions (Assay Designs). Each bar represents the mean ± standard error of triplicate values. The data are from one experiment and are representative of three independent experiments. Asterisks denote a statistically significant difference between untreated and ET-treated cells (p < 0.05, ANOVA followed by the Student’s t-test).